ATG fect solution+ATGfeed medium

概述（Summary）

产品描述（Description）

ATG fect solution 是一种基于阳离子聚合物的优异转染试剂。它可应用于多种细胞系的各种规模的转染。与市场上其他的转染试剂相比，ATG fect solution 能够高效率转染多种细胞系，包括贴壁细胞和悬浮细胞，尤其对于悬浮细胞（如 293-F、CHO-S 等）表现出卓越的性能，同时也具有具有较低的细胞毒性。 ATGfect solution 是用于瞬时和稳定转染的重组蛋白生产的理想选择。

储存条件（Storage）

请于-20 °C 保存，保质期一年。

运输方式（Shipping）

蓝冰运输

Note

For research use only .

状态（Form）

Liquid

使用方法（Standard Operating Procedure）

PROTOCOL FOR SUSPENSION CELLS:
Preparation: 18 to 24 hours before transfection, seed cells at 1.0 x 106 per mL of culture. Sample Transfection.Protocol (1000 mL culture in 3L shake flask).

1. Prepare ATG fect solution-DNA transfection mixture (order is critical):
(1) Add 500 µg of plasmid DNA into100 mL FBS-free medium.
(2) Briefly mix/vortex solution.
(3) Add 1.5 mL of ATGfect solution to mixture.
(4) Vortex solution for 5 seconds.
(5) Let solution sit for 20 minutes in hooded environment to allow ATG fect solution -DNA complexes to form.
(6) Gently mix solution by pipetting up and down 3 times.

2. Add entire transfection solution to 1000 mL of cell suspension culture.

3. Return cell suspension to incubator.

4. Recombinant protein can be analysed 4-6 days after transfection.

Note: The above operation is for reference only. Please explore the ratio of transfection reagent to DNA according to your experimental needs to find the best transfection conditions.